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KMID : 0368419860290030197
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Journal of Plant Biology
1986 Volume.29 No. 3 p.197 ~ p.205
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Protoplast Culture in Five Cultivars of N. tabacum L. by Modified Murashige and Skoog Medium
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Abstract
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Leaf mesophyll protoplasts from five cultivars of tobacco (N. tabacum L.) were cultured. The protoplasts did not survive in culture medium containing Murashige and Skoog inorganic salts for over 6 days. NH_4NO_3 and FeSO_4·Na_2EDTA concentration of Murashige and Skoog medium were toxic in tobacco leaf mesophyll protoplast culture. Therefore we investigated optimum condition in Murashige and Skoog medium. High plating efficiency was obtained by reducing the concentrations of NH_4NO_3 and FeSO_4·Na_2EDTA to 1/3 and 1/10, respectively, on the supplemented with 5 μM IAA, 0.5 μM 2,4-D, 5 μM BAP. Plants were regenerated from protoplast-derived calluses.
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